Sign in →

Test Code ANA Anti-Nuclear Antibody (ANA)

Clinical Indications

Test Method: Immunofluorescence Assay (IFA) utilizing Hep-2000 cells

The detection and semi-quantitation of antinuclear antibodies (ANA) are an important aid in the diagnosis of systemic rheumatic diseases such as systemic lupus erythematosus (SLE), mixed connective tissue disease (MCTD), Sjogren’s syndrome (SS), progressive systemic sclerosis (PSS), polymyositis, and rheumatoid arthritis (RA).

Additional Test Codes

Epic Order Code: Anti-Nuclear Antibody (ANA)
Sunquest Order Code: ANA
Px Order Code: LAB2068

Specimen Collection Type

Serum
Preferred: Gold top (SST gel separator)
Acceptable: Red top (serum tube)

Allow blood to clot at room temp (18-24°C). Serum should be separated from the clot ASAP to minimize hemolysis.

Minimum Collection Volume

0.3 mL

Reference Range

<80

Critical Value

N/A

Interpretation

Negative: <1:80
Positive: >1:80

ANA titer is performed from 1:80 to 1:1280 dilutions. Pattern reported when positive.

Some patients without clinical evidence of autoimmune disease or systemic rheumatic disease may have a detectable level of ANA. ANA may also be detectable following viral illness, in chronic infection, and in patient treated with certain medications.

Rejection Criteria

Gross hemolysis
Gross lipemia

Specimen Availability for Add-on Testing

3 days

Specimen Stability

7 days at 2-8°C
14 days at -20°C

Day(s) Performed

Monday through Friday

Performing Lab- Department

TUKHS-BH-Special Chemistry

 

CPT Code

86038

LOINC Code

8061-4

Test Alias

N/A

Additional Information / Notes

N/A