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Test Code BRAFN BRAF - Next-Generation Sequencing

Clinical Indications

N/A

Additional Test Codes

Epic Order Name: BRAF SINGLE NGS
Sunquest Order Code: BRAFN
Epic Px Code: LAB8173

Specimen Collection Type

Blood: EDTA (lavender-top) tube, 3.0 mL (Minimum 0.5 mL);
Bone Marrow Aspirate: EDTA (lavender-top) tube, 3.0 mL (Minimum 0.5 mL);
FFPE tissue: 5 to 10 slides, each up to 10 ?m thick plus one additional H&E reference slide with tumor tissue marked.

Minimum Collection Volume

Blood: 0.5 ml;
Bone Marrow Aspirate: 0.5 ml;
FFPE: 5 unstained slides + 1 representative H&E

Additional Information / Notes

Useful for predicting TKI inhibitor response in melanomas, colorectal cancers, gastrointestinal stromal tumors (GISTs), and inform diagnoses in gliomas and hairy cell leukemias.

Reference Range

An interpretive report will be provided

Critical Value

N/A

Interpretation

An interpretive report will be provided

Rejection Criteria

Frozen, severely hemolyzed, or clotten specimen. FFPE tumor specimen with a tumor percentage < 5 %, or with otherwise inadequate cellular content.

Specimen Availability for Add-on Testing

Only for additional CMOL testing; additional sample may be required, see test sample requirements and stability.

Specimen Stability

Blood/Bone Marrow Aspirate: 96 hrs at 4°C;
FFPE: n/a.

Day(s) Performed

9:00 AM – 5:00 PM, Monday through Friday; varies

Performing Lab - Department

Clinical Molecular Oncology Laboratory

CPT Code

81210

LOINC Code

N/A

Test Alias

N/A