Test Code DNA Anti-DNA Double Strand
Clinical Indications
Test Method: Crithidia luciliae Immunofluorescence Assay (IFA)
The detection and quantitation of anti-double-stranded DNA (dsDNA) is considered diagnostically and therapeutically helpful in patients suspected of or known to have systemic lupus erythematosus (SLE). High levels of anti-dsDNA are most often seen in active SLE.
Additional Test Codes
Epic Order Code: Anti-DNA Double Strand
Sunquest Order Code: DNA
Px Order Code: LAB2059
Specimen Collection Type
Serum
Preferred: Gold top (SST gel separator)
Acceptable: Red top (serum tube)
Allow blood to clot at room temp (18-24°C). Serum should be separated from the clot ASAP to minimize hemolysis.
Minimum Collection Volume
0.3 mL
Reference Range
<10
Critical Value
N/A
Interpretation
Negative: <1:10
Positive: >1:10
A dsDNA titer is performed from 1:20 to 1:1280 dilutions.
A negative result does not exclude a diagnosis of SLE. Positive results may occur in patients with diseases other than SLE.
Rejection Criteria
Gross hemolysis
Gross lipemia
Specimen Availability for Add-on Testing
3 days
Specimen Stability
7 days at 2-8°C
14 days at -20°C
Day(s) Performed
Monday through Friday
Performing Lab- Department
TUKHS-BH-SPECIAL CHEMISTRY
CPT Code
86215
LOINC Code
31348-6
Test Alias
N/A
Additional Information / Notes
N/A