In order to provide the best results, either answer the order entry questions or provide the required information using the Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information (T595).

Specimen Required

Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Do not collect specimen after amniocentesis as this could affect results.

2. Centrifuge and aliquot serum into plastic vial within 2 hours of collection.

Additional Information:

1. Collect blood between 15 weeks, 0 days and 22 weeks, 6 days.

2. Initial or repeat testing is determined in the laboratory at the time of report and will be reported accordingly. To be considered a repeat test for the patient, the testing must be within the same pregnancy and trimester, with interpretable results for the same test, and both tests are performed at Mayo Clinic.

Forms

1. Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information (T595) is required

2. If not ordering electronically, complete, print, and send a General Request (T239)

Secondary ID

113382

Useful For

Prenatal screening for open neural tube defect

Special Instructions

Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information

Method Name

Two-Site Immunoenzymatic (Sandwich) Assay

Reporting Name

AFP Single Marker SCRN, Maternal, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type	Temperature	Time
Serum	Refrigerated (preferred)	14 days
	Frozen	90 days
	Ambient	7 days

Reject Due To

Gross hemolysis	Reject
Gross lipemia	OK
Gross icterus	OK

Reference Values

NEURAL TUBE DEFECTS:

An alpha-fetoprotein (AFP) multiple of the median (MoM) <2.5 is reported as screen negative.

AFP MoM ≥2.5 (singleton and twin pregnancies) are reported as screen positive.

An interpretive report will be provided.

Day(s) Performed

Monday through Friday

Report Available

4 to 6 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82105

LOINC Code Information

Test ID	Test Order Name	Order LOINC Value
MAFP1	AFP Single Marker SCRN, Maternal, S	48802-3

Result ID	Test Result Name	Result LOINC Value
7058	Recalculated Maternal Serum Screen	32399-8
113146	Results Summary	32399-8
113147	Neural tube defect risk estimate	48803-1
10351	AFP	83073-7
113148	AFP MoM	23811-3
10356	INTERPRETATION	49092-0
10357	RECOMMENDED FOLLOW UP	80615-8
10248	Additional comments	48767-8
3009	Specimen collection date	33882-2
7823	Maternal date of birth	21112-8
7834	Calculated age at EDD	43993-5
26717	Maternal Weight	29463-7
26718	Maternal Weight	29463-7
IDD	Insulin dependent diabetes	44877-9
RACE1	Patient race	21484-1
SMKNG	Current cigarette smoking status	64234-8
10054	EDD by U/S scan	11781-2
7203	GA on collection by U/S scan	11888-5
7753	EDD by LMP	11779-6
7204	GA on collection by dates	11885-1
7830	GA used in risk estimate	21299-3
MULTF	Number of Fetuses	55281-0
CHOR_	Number of Chorions	92568-5
IVFP	IVF pregnancy	47224-1
PRNTD	Prev Pregnancy w/ Neural Tube Defect	53827-2
PTNTD	Patient or father of baby has a NTD	53827-2
INTL	Initial or repeat testing	77202-0
DRPHN	Physician Phone Number	68340-9
10358	GENERAL TEST INFORMATION	62364-5

Laboratory Services Directory Additional Information:

Aliases:

Alpha-Fetoprotein (AFP), Single Marker Screen, Maternal, Serum

AFP Maternal Screening

AFP Neural Tube Defects

Maternal Screening, AFP Single Marker